Antibiotic Resistant Infection of the Bacterial Group ESKAPE

Abstract

Objectives: The antibiotic resistant infection of six bacterial species have
been designated as 'Red Alert' human pathogens, which are responsible for
2/3 of all hospital acquired infection, they are grouped under acronym
ESKAPE which include: 1- Enterococcus faecim or faecalis, 2-
Staphylococcus aureus, 3- Klebsiella pneumoniae, 4- Acinetobacter
baumannii, 5- Pseudomonas aeruginosa, 6- Enterobacter spp. For the first
time as group in Mosul and Duhok / Iraq.
Methods: Three hundred and thirty-two samples from different sources as
Urine, Blood, Wounds, Burn, High Vaginal Swab (H.V.S.), Throat
infection, Nose infection, Cerebrospinal fluid (C.S.F.) and Sputum were
collected from hospital patients in city of Mosul and Duhok in Iraq. These
samples were cultured on blood and Macconkey's agar. The bacterial
colonies were purified and identified to species level using morphological,
Biochemical tests , API and confirmed by Vitek 2 System and antibiotic
sensitivity was carried out using Vitek 2 System.
Results: Our results showed that 73 isolates 21.98 % were gave growth
only and 34.25 % were gram positive and 65.75 % were gram negative and
the most isolated number from burns 50 % and the lowest isolated number
from C.S.F. 7.69 %.
Fourty eight species were Kleb. pneumoniae(13), A. baumannii(12),
Pseudo. aeruginosa(11) and E. cloacae(12) and Twenty five species were
Staph. aureus(17) and E. faecalis(8).
The result also showed the most bacterial species within ESKAPE group
were resistant for almost antibiotics used under study.
Conclusion: We were isolated ESKAPE bacterial species from acquired
hospital infection and they comprise the majority of antibiotic resistance
seen in hospital which diagnosed them by Vitek 2 System.