Isolation and Diagnosis of Staphylococcus lentus from Different Operation Theater Hospitals

Abstract

Objectives: Hospital environment play critical role in nosocomial
infection, because this environment contains various microorganisms. A
frequency of increased pathogenic bacteria in the environment of the
hospital is associated with a surroundings rise in different types of
nosocomial infections.
Methods: A total of 500 samples were collected from environmental
samples and clinical samples were distribution on Basra hospitals during
the period from October 2016 to February 2017. All isolates were subjected
to the cultural, microscopical ,biochemical examination and identification
by 16S ribosomal DNA (16S rDNA) and Sequencing of 16S rDNA.
Results: It was found that 204 (40.8%) of the total bacterial agents in all
surgical theaters with a significant statistical superiority in the prevalence
of positive bacteria 187 (91.66%)in different surgical theaters. The study
included the diagnosis of 53 isolates (91.37%) Gram positive cocci
formulations giving 47 isolates (81.03%) of Staphylococcus species as
follows: 17 isolation of Staphylococcus lentus (29.31%). Staphylococcus
lentus was Sensitive to Benzyl penicillin, Oxacilin, Gentamicin,
Teicoplanin, Tigecycline, Trimethoprin and Nitrofurantion,Levofloxacin,
Rifampicin. While S.lentus was Resistant to Erythromycin, Levofloxacin,
Vancomycin, but S. lentus was Intermediate to Moxifloxacin
Clindamycin, Teteacycline , Fosfomycin.
Conclusion: The emergence of a new type of bacteria Staphylococcus
lentus which has not been isolated from hospitals previously.
Staphylococcus lentus are considered as a pathogenic bacteria and it has
the ability to pathology as well as multiple resistance to antibiotics.