Study of The Toxic Effect of Tartrazine Dye on Some Biochemical Parameters in Male Albino Rats

Abstract

Objectives: The present study aimed to demonstrate the toxic effects of tartrazine pigment on some biochemical parameters related to liver and kidney functions. Methods: The experiment was conducted on male rats aged 10-15 weeks with a weight of 190-250g. The experimental animals were subjected to suitable laboratory conditions at a temperature of 20-25 degrees Celsius. The feed and water were given freely during the period of the experiment. The animals were divided into a negative control group that included nine Animals that were dosed with a physiological salt solution throughout the trial period. The positive control group included nine animals who were dosed orally with tartrazine dye at a concentration of 400 mg / kg / bw for a period of 30 days. Results: The results of the study showed that dosing animals with tartrazine at a concentration of (400mg / kg / bw) for 30 days led to a significant increase (P <0.05) in the levels of liver enzymes in the blood serum compared to the control group,This was accompanied by a marked decrease in the concentration of blood electrolytes due to the effect of accumulation of products the oxidative stress resulting from the effect of the dye on the activity of the enzyme Na + / K + ATPase, which plays an important role in regulating the concentrations of electrolytes inside and outside the cell, Noting that there has been a marked increase in the biochemical parameters, which include urea, creatinine and uric acid, which are considered among the basic criteria for determining the rate of glomerular filtration and tube excretion of the kidneys. Conclusion: The results of the current study showed that treating male rats with tartrazine dye (400mg / kg / bw) for a period of 30 days led to significant changes in the biochemical parameters studied when compared with the control group.