Effect Extraction of Nigella Sativa and Malricaia chamoilla on Urinary Tract Infection
Main Article Content
Abstract
Objectives: The proposed of current study determined effectiveness of extracts for Niglle sative and Malricia chamoilla on the Urinary tract infection Bacteria like gram’s positive Staphylococcus aureus, streptococcus faecalis and gram’s negative bacteria Escherichia coli, Klebsiella pneumonia. Methods: For obtain of crude extract of peat solvents had been used are hot, cold water and ethanol , four types of Pathogenic bacteria which tested effectiveness of plant extract and two gram’s positive pathogenic bacteria Staph. aureus, strept. faecalis and gram’s negative bacteria E. coli, K. pneumonia isolated from urinary tract infection (UTI) patient to detection the most active solvent extraction, and used various methods of extraction alcohol, hot water and cold water, then was used of many dilutions to each powder of extraction , to determine the inhibition zone on the plats using well method and measuring the inhibition zone to determine the sensitivity and resistance for UTI infection bacteria. Results: The current study results showed best method of extraction solvent is ethanol that appear through inhibition zone of pathogenic bacteria reach to 50mm in diameter in some case near the inhibition zone of antibiotic diameter. The result also showed there is no effect when used M. chamoilla extract alone on both gram’s negative and positive and there is few effect when used N. sativa extract alone on both gram’s negative and positive but when mixed the plant extraction of both plant N. sativa and M. chamoilla the effect of plant extraction on growth of pathogenic bacteria by diameter of inhibition zone increased reach 50mm, 45mm on gram’s positive bacteria and this result near the inhibition zone of antibiotic while in gram’s negative bacteria reach in range 45mm, 40mm and 35mm diameter of inhibition zone. Result showed there is no effect in cold water extraction. Conclusion: The best method of extraction ethanol extraction and most effectiveness plant extraction when mixed the of N. sativa and M. chamoilla produce antimicrobial activity against gram’s positive bacteria and gram’s negative bacteria when used ethanol extracted method.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Carvalho A.F., Silva D.M., Silva T.R., Scarcelli E. and Manhani M.R. "Avaliação da atividade antibacteriana de extratos etanólico e de ciclohexano a partir das flores de camomila (Matricaria chamomilla L.)". Rev. Bras. Pl. Med., Campinas. 2014; 16 (3): 521-526. DOI: https://doi.org/10.1590/1983-084X/12_159.
Astal Z.E.L. " Increasing ciprofloxacin resistance prevalent Urinary tract bacterial isolates in Gaza strip". Palestine J. of Bio Technology. 2005; 2005(3): 238-241. DOI: 10.1155/JBB.2005.238.
Duke J.A. "Medical Book". Culinary Herbs. Apotpourri , New York. 1985; PP 26-30.
Aljanaby A.A.J. "In vitro antibacterial activity of an aqueous extracts of Matricaria chmomilla flowers against pathogenic bacteria isolated from pregnant women with urinary tract infection". Biomed. Res. 2018; 29 (11): 2395- 2400.
Romha G., Admasu B., Hiwot Gebrekidan T., Aleme H. and Gebru G. "Antibacterial activities of five medicinal plants in ethiopia against some human and animal pathogens". Evid Based Complement Alternat. Med. 2018; 2018(8): 1-10. DOI: 10.1155/2018/2950758.
Aljanaby A.A.J. and Alhasnawi H.M.R.J. "Phenotypic and molecular characterization of multidrug resistant Klebsiella pneumoniae isolated from different clinical sources in Al-Najaf Province-Iraq". Pak J Biol Sci. 2017; 20(5): 217-232. DOI: 10.3923/pjbs.2017.217.232.
Ghouri F., Hollywood A. and Ryan K. "A systematic review of non-antibiotic measures for the prevention of urinary tract infections in pregnancy". BMC Pregnancy Childbirth. 2018; 18(1): 99. DOI: 10.1186/s12884-018-1732-2.
Dhanasekaran S. "Alteration of Multi-drug Resistance Activities by Ethanolic Extracts of Nigella sativa Against Urinary Pathogens". Int. J. Pharmacol. 2019; 15(8): 962-969. DOI: 10.3923/ijp.2019.962.969 .
Hayatdavoudi P., Rad A.K., Rajaei Z. and Hadjzadeh M.R. "Renal injury, nephrolithiasis and Nigella sativa: A mini review". AJP. 2016; 6( 1): 1-8.
Tavakoli A., Mahdian V., Razavi B.M. and Hosseinzadeh H. "Review on clinical trail of black seed (Nigella sativa) and it’s active constituent thymoquinone". J. Pharmacopuncture. 2017; 20(3): 179- 193. DOI: 10.3831/KPI.2017.20.021.
Hammadi A.A., Abdullah J.A. and Abed H.A. "Study of inhibition efficiency of peat against burn infection bacteria". Sci. J. Med. Res. 2017; 1( 2): 78 – 81. DOI:10.37623/SJMR.2017.1209.
Sci. J. Med. Res. Vol. 4, Issue 16, pp 118- 124 Autumn 2020 123
.
Abdullah J.A., Hammadi A.A., Hakem R., Hatef Z. and Hussein N. "Study effect of plant extraction for Cuscuta europaea (Dodder) against two species of bacteria Staphylococcus aureus and Escherichia coli" . J Contemp Med Sci. 2016; 2( 8): 133–137. DOI: 10.22317/jcms.2016126.
Shalekenov B.U., Bissekenova A.L., Ramazanova B.A., Adambekov D.A. and Shalekenov S.B. "Species composition and molecular-genetic characteristics of antibioticresistant strains of gram negative bacteria isolated from patients of a hospitals urology department". Urologiia. 2018; 1: 77-83.
Pailhoriès H., Cassisa V., Chenouard R., Kempf M., Eveillard M. and Lemarié C. "Staphylococcus saprophyticus: Which beta-lactam?". Int. J Infect Dis. 2017; 65: 63-66. DOI: 10.1016/j.ijid.2017.10.001.
Kalas V., Hibbing M.E., Maddirala A.R., Chugani R., Pinkner J.S., Mydock-McGrane L.K., Conover M.S., Janetka J.W., Hultgren S.J. "Structure-based discovery of glycomimetic FmlH ligands as inhibitors of bacterial adhesion during urinary tract infection". Proc Natl Acad Sci USA. 2018; 115 (12) E2819-E2828. DOI: https://doi.org/10.1073/pnas.1720140115.
Awaad A.A., El-Meligy R.M., Zain G.M., Safhi A.A., A Qurain N.A., Almoqren S.S., Zain Y.M., Sesh Adri V.D. and Al- Saikhan F.I. "Experimental and clinical antihypertensive activity of Matricaria chamomilla extracts and their angiotensin-converting enzyme inhibitory activity". Phytother Res. 2018; 32(8):1564-1573. DOI: 10.1002/ptr.6086.
Blumenthal M. "The complete German commission and monographs. Therapeutic guide to herbal medicines". Integr Med Communications, Austin, Texas 2000.
Achterrath-Tuckermann U., Kunde R., Flaskamp E., Isaac O. and Thiemer K. "Pharmacological investigations with compounds of chamomile. V. Investigations on the spasmolytic effect of compounds of chamomile and Kamillosan on the isolated guinea pig ileum". Planta Med. 1980; 39(1): 38-50. DOI: 10.1055/s-2008-1074901.
Gadisa E., Weldearegay G., Desta K., Tsegaye G., Hailu S., Jote K. and Takele A. "Combined antibacterial effect of essential oils from three most commonly used Ethiopian traditional medicinal plants on multidrug resistant bacteria". BMC Complement. Altern. Med. 2019; 19(24): 1-9. DOI: https://doi.org/10.1186/s12906-019-2429-4.
Dhanasekaran S. "Alteration of Multi-drug Resistance Activities by Ethanolic Extracts of Nigella sativa Against Urinary Pathogens". Int. J. Pharmacol. 2019; 15 (8): 962-969. DOI: 10.3923/ijp.2019.962.969.
Emeka L.B., Emeka P.M. and Khan T.M. "Antimicrobial activity of Nigella sativa L. seed oil against multi-drug resistant Staphylococcus aureus isolated from diabetic wounds". Pak. J. Pharm. Sci. 2015; 28(6): 1985-1990.
Anthony M.P., Burrows J.N., Duparc S., Moehrle J.J. and Wells T.N. "The global pipeline of new medicines for the control and elimination of malaria". Malar. J. 2012; 11(10): 1186/1475-2875-11-316.
Cantas L., Shah S.Q.A., Cavaco L.M., Manaia C. and Walsh F., et al. "A brief multi-disciplinary review on antimicrobial resistance in medicine and its linkage to the global environmental microbiota". Front. Microbiol. 2013; 4(10): 96. DOI: 10.3389/fmicb.2013.00096.
Singh S., Das S.S., Singh G., Schuff C., de Lampasona M.P. and Catalan C.A.N. 2014. "Composition, in vitro antioxidant and antimicrobial activities of essential oil and oleoresins obtained from black cumin seeds (Nigella sativa L.)". Bio Med Res. Int. 2014; 2014: 1-10. DOI: https://doi.org/10.1155/2014/918209.
Uzair B., Hameed A., Nazir S., Khan B.A., Fasim F., Khan S. and Menaa F. "Synergism between Nigella sativa seeds extract and synthetic antibiotics against Mec A gene positive human strains of Staphylococcus aureus". Int. J. Pharmacol. 2017; 13(8): 958-968. DOI: 10.3923/ijp.2017.958.968.