Isolation and Identification of Nosocomial Pathogen Acinetobacter baumannii From Al-Hussien Medical City in Karbala

Abstract

Objectives: Acinetobacter baumannii is among the most opportunistic pathogenic nosocomial associated with the hospital settings infections such as bacteremia, meningitis and UTIs . The growing emergence of these organisms' multidrug resistance demonstrates the need to identify the spread of these bacteria and their resistance to the antibiotics which are used in Iraqi hospitals. Methods: A total of 135 clinical samples from patients (urine, blood, CSF , swabs of wound and burns)and swabs of medical devices from ICU and burn units were collected in AL-Hussein Medical City in Karbala during the period from July to October 2018. Furthermore, Polymerase Chain Reaction Technique (PCR) was performed to identify the ?-Lactamase enzyme encoding resistance genes include all of bla oxa -51 and bla ampc gene. Results: 15 isolates (11.11%) of Acinetobacter baumannii have been described, with swabs of burns showing a high percentage of A. baumannii isolates (17.85%) Whereas other samples were variable in percentage. Susceptibility of 15 A.baumannii isolates to 16 different antimicrobials has been evaluated, the results showed that five isolates were multi drug resistance (MDR) and the others were extensive drug resistance (XDR). The PCR analysis showed that all isolates have bla oxa-51 gene while 11 isolates have bla ampc gene. Conclusion: It was concluded that there is a high prevalence of Acinetobacter baumannii isolates in the burns unite and that all isolated bacteria characterized by their high antibiotic resistance, this resistance was confirmed by the detection of ?-Lactamase enzymes which are encoded by the resistance genes bla oxa-51 gene and bla ampc gene.