Effect of Cassia senna Fruit Hexane Extract as Antibacterial Against Urinary Tract Infection Pathogens and Antioxidant Activity

Abstract

Objectives: This study investigate the role of hexane extract of Cassia senna fruits as antibacterial against urinary tract infection UTI pathogens and antioxidant activity. Methods: The chemical composition of extract was performed by GC-MS Analysis. the antibacterial potential was achieved using agar well diffusion method and Minimum inhibitory concentration method also determination of human red blood cells (RBCs) cytotoxicity whereas antioxidant performed by DPPH assay. Results: The results of GC-MS showed linolaidic acid 25.08%, linoleic acid ethyl ester 20.04%, beta-sitosrol 14.8%, gamma- sitosterol 13.37%, hexadecanoic acid ethyl ester 13.04%, tetracosane 9.16%, Stigmasterol 6.73%, Lupeol and Betulin area was 5.66%. the extract exhibit antibacterial activity against UTI pathogens with excellent and broad spectrum activity as compared with antibiotics with no toxic effect against human(RBCs). the highest inhibition zone was 50 mm of Staphylococcus pseudintermedius and the lowest 18 mm for proteus vulgaris with MIC ranging between less than 1 and 1 mg. From the analysis of antioxidant potential of Cassia Senna hexane fruit extract revealed high inhibition DPPH percent 91% at high concentration 1000 ul/ml as well as ascorbic acid. Conclusion: This study highlights that hexane extract of Cassia senna fruit has potential components which elicited their biological activities against UTI pathogens and antioxidant.