Immunohistochemical Staining of Epstein - Barr Virus LMP-1 Expression in Patients with Lymphoma

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Salwa S. Shihab
Hayder Abdulhussein- Al-Hmudi
Sadeq K. Ali. Al. Salait


Background: Epstein-Barr virus (EBV) is a common herpes virus that can be linked to a variety of infections and cancers. Some kind of these diseases are good defined and known, others are poorly defined and weakly understood. Objectives: The present study was aimed to study the expression of Epstein Barr virus (EBV) encoded oncoprotein LMP1 in the lymphoma patients. Methods: A 67 blood samples of both sexes with the age range 3 to 93 years’ old were collected from patients with malignant lymphoid solids. The nested PCR protocol it was used to amplify two DNA fragment of EBV. The immunohistochemical staining was used for detection of LMP1 proteins. Results: EBV-DNA was detected in 24/67(35.82%) patients. The positive results of EBV-DNA were distribution among the studied groups of patients as follow: NHL group [20, 83.33%; 11(55%) females and 9(45 %) males], HL group [3, 12.5%; 2(66.66%) females and 1(33.34%) males] and [1, 4.17% (male only)] for BL. A 5/16 (31.25%) samples revealed positive staining of LMP1 protein. Conclusion: we concluded that the positive EBV paraffin embedded samples was showing strong immunostaining for LMP1 protein.


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Review Article