Detection H. pylori Infection by BabA Gene From Clinical Isolate in Karbala City, Iraq

Abstract

Objectives: The goal of this study is focused on the effect of BabA was investigated as being responsible for the adhesion of bacteria to epithelial tissue to make colonization and using this gene is effective methods to diagnosis this bacteria. Methods: The clinical samples was taken during the period from August 2018 to March 2019 for 58 men and 54 women, their ages between 10-80 years old. Detection of the H. pylori infection by demonstration of BabA gene. Out of 112 patients suffering from dyspepsia attending Al- Husain medical city / digestive system diseases center, Karbala city. Detection of H. pylori infection from gastric biopsies by many methods culturing bacteria from the clinical samples on selective media Columbia agar, morphological test likes gram’s stain, biochemical including urease, oxidase, catalase, Oxoid biochemical identification system and API CAMPY identification test and molecular identification. Results: It was found high infection rates of H. pylori in Karbala city 87 (71.3%) patients were positive H. pylori infection, the females had more frequent of H. pylori infection than males, and the infection was more in younger ages. The distribution of H. pylori infection according to age group and gender revealed that the majority of infected patients found in younger ages and then the curve declined after forties. The total males/ females ratio was 0.89/ 1 of the infected patients. The current study showed that the H. pylori patients with various pathologies cases as follow: gastritis in 48 case, peptic ulcer 19 case, gastric hemorrhagic 8 case, gastric pain 5 case and gastric cancer 3 case. There are many tests using to detect H. pylori infection. The highest percentage of these was PCR, especially when using more than one gene multiplex PCR, then urease test as rapid local test , then culturing that used especial requirements to grow bacteria which was considered fastidious and then gram’s stain. Also this study showed a very important relation between H. pylori infection and iron deficiency anemia due to the iron was utilized by bacterial colonization during infection leading to malabsorption of iron. Conclusion: The most frequent medical cases associated with H. pylori infection are gastritis , peptic ulcer, gastric hemorrhagic and then gastric cancer respectively. The H. pylori had high rates in Karbala city population and female patient are more frequently infection, and the infection was more in younger ages. There were many methods used to diagnosis H. pylori infection, PCR sensitive one and specialy when used more one gene duplex PCR. There are important relations between H. pylori infection and iron deficiency anemia.