Molecular Diagnosis of Bacteria Isolated from Colorectal Cancer Patients with Potential Use as A Diagnostic Tool for The Disease

Abstract

Background: The human gut environment is colonized by a vast and diverse microbial community, vastly outnumbering the cells of the human body itself, many of which remain unidentified Microorganisms inhabit nearly all areas of the human body, possessing a diverse array of genes. These genes can interact with, alter, or deactivate numerous human genes, particularly within colon cells. “..

Aim of study: Isolation and molecular characterization of intestinal bacteria associated with colorectal cancer and their role in tumorigenesis.

Methods:137 different samples were collected from infected individuals. The samples included blood, stool, and biopsy taken from the endoscopy unit inside Al-Sadr Teaching Hospital in Basra Governorate during the period from March 2022 to December 2023.

Results: The bacteria associated with colorectal cancer patients were isolated and molecularly characterized by 16S rRNA sequencing. 27 different species were identified, including enteric genera, rare genera, and anaerobic genera.

Conclusion: The study identified bacteria such as Fusobacterium nucleatum, E. coli, Salmonella enterica and other species associated with the disease. These bacteria contribute to the development of the tumor and can serve as diagnostic markers for its detection.