The effect of Diabetes mellitus (DM) induction on the Structure of Skeletal Muscle in experimental rats and the protective role of arginine on (DM) complications (Ultrastructural Study )
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Abstract
Objective: The aim of this study was detect the ultrastructural alterations of
gastrocnemius skeletal muscle related to diabetic rats and control was
studies , role of arginine as protective agent against (DM) complication was
clarified at each period (15,30,45) days post induction.
Methods: Thirty rats divided into two groups diabetic and nondiabetic
group , the diabetic group are subdivided to two groups , the first
given tap water , the second given tap water containing L-arginine
dissolved in the drinking water.
Results: Ultrathin sections from skeletal muscles biopsies showed
myofibrils with sever contraction , atrophied and less regular , disorganized
Z-line , variable degree of axonal atrophy , congested capillaries,
post(30)days, abnormal myofibrils, changes in mitochondrial position and
showed subsarcolemma location, large lipid droplets , distoration of
sarcoplasmic reticulum , lamellar formation of collagen, also post (45) day,
revealed to irregular T-tubules , absence of Z-line , degenerated myofibrils ,
muscle fibers fragments , fibers splitting . The study determined the effect
of arginine on skeletal muscle related to diabetes rats at each period
(15,30,45 ) days , results recorded condensed myofibrils,more regular,well
developed, regularZ-line and clear lamellar structure of collagen , the
axoplasm filled with mitochondria , normal schwann cell nuclei and
deposition of glycogen granules in the interistitial space, oriented Z-line ,
myotube with normal myonuclei and the arrangement of T-tubules more
obvious , the results showed that the arginine resulted in minimized the
histological changes and reduced STZ-induced ( DM) and its toxic effect
that seen in rats with (DM).
Conclusion: Strong association between ultrastructural changes of diabetic
skeletal muscles and their innervation was established and the results
reffered to arginine as protective, bioactive agent reduced myopathy ,
neuropathy of diabetic muscles.
gastrocnemius skeletal muscle related to diabetic rats and control was
studies , role of arginine as protective agent against (DM) complication was
clarified at each period (15,30,45) days post induction.
Methods: Thirty rats divided into two groups diabetic and nondiabetic
group , the diabetic group are subdivided to two groups , the first
given tap water , the second given tap water containing L-arginine
dissolved in the drinking water.
Results: Ultrathin sections from skeletal muscles biopsies showed
myofibrils with sever contraction , atrophied and less regular , disorganized
Z-line , variable degree of axonal atrophy , congested capillaries,
post(30)days, abnormal myofibrils, changes in mitochondrial position and
showed subsarcolemma location, large lipid droplets , distoration of
sarcoplasmic reticulum , lamellar formation of collagen, also post (45) day,
revealed to irregular T-tubules , absence of Z-line , degenerated myofibrils ,
muscle fibers fragments , fibers splitting . The study determined the effect
of arginine on skeletal muscle related to diabetes rats at each period
(15,30,45 ) days , results recorded condensed myofibrils,more regular,well
developed, regularZ-line and clear lamellar structure of collagen , the
axoplasm filled with mitochondria , normal schwann cell nuclei and
deposition of glycogen granules in the interistitial space, oriented Z-line ,
myotube with normal myonuclei and the arrangement of T-tubules more
obvious , the results showed that the arginine resulted in minimized the
histological changes and reduced STZ-induced ( DM) and its toxic effect
that seen in rats with (DM).
Conclusion: Strong association between ultrastructural changes of diabetic
skeletal muscles and their innervation was established and the results
reffered to arginine as protective, bioactive agent reduced myopathy ,
neuropathy of diabetic muscles.
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How to Cite
Hassani, M. k., & Ibrahim, M. K. (2018). The effect of Diabetes mellitus (DM) induction on the Structure of
Skeletal Muscle in experimental rats and the protective role of
arginine on (DM) complications (Ultrastructural Study ). Scientific Journal of Medical Research, 2(07), 113–123. https://doi.org/.
Section
Review Article