Isolation of methicillin-resistant Staphylococcus aureus from burn centers and evaluation of antimicrobial efficacy, in Faisalabad, Pakistan

Abstract

Objectives: The present study was conducted for the detection of MRSA
infections in burn wards and for evaluation of different antibiotics efficacy.
Methods: A total of 100 samples were collected from burn ward of Allied
hospital Faisalabad, Pakistan. Then all samples were cultured on Mannitol
salt agar, S-110 agar and then selective biochemical tests were performed.
Antibiotic sensitivity was checked by using different beta-lactam antibiotics
including methicillin, and vancomycin, linezolid, amoxicillin along with
clavulanic acid (ESBL inhibitor) and some others, according to criteria of
Clinical Laboratory Standard Institute (CLSI), to find out better
antimicrobial therapy for burn patients.
Results: A total of 28 (66.66%) isolates of MRSA were detected and
remaining 14 (33.33%) were MSSA, out of 42 isolates of S. aureus. The
results showed that highest risk of MRSA prevalence was associated with
burn patients and had a percentage positivity of 66.66% among burn
patients of Allied hospital, Faisalabad.
Conclusion: It was observed that maximum resistance was found against
beta-lactam drugs especially oxacillin (98%), but maximum level of
sensitivity was observed against linezolid (95%) and vancomycin (99.99%),
means that, these are more effective drugs for the treatment therapy of burn
patients.