Etiology and antimicrobial resistance patterns of Extended Spectrum Beta Lactamases (ESBL) producing E. coli from urinary tract infections in Tehsil Head Quarter (THQ) Hospital, Burewala, Pakistan
Main Article Content
Abstract
Objectives: The present study was conducted to isolate Extended Spectrum
Beta-Lactamases(ESBL) producing strain of E. coli causing UTIs in
hospitalized patients and to evaluate antimicrobial resistance pattern of
most commonly used antibiotics in treatment therapy, in Tehsil headquarter
hospital in Burewala.
Methods: A total of 150 samples were collected from Patients diagnosed
with urinary tract infections (UTIs), from Tehsil Head Quarter (THQ)
Hospital, Burewala, Pakistan. All samples were streaked on specific media,
MacConkey agar where positive isolates of E. coli showed pink colored
colonies, further confirmation was done by performing some biochemical
tests. ESBL detection of E. coli isolates was confirmed by performing
Double Disc Synergy Test (DDST). In the end antimicrobial resistance
pattern was checked by using some commonly used antibiotics such as
ampicillin, amoxi-clavulanic acid combination, ciprofloxacin, norfloxacin,
TMP-SMX, ceftriaxone and nitrofurantoin and results were observed.
Results: A total of positive 58 samples for E. coli were detected and out of
58 samples, 43 samples were positive for ESBL-E. coli strain and rests
were non-ESBL producers. The results showed higher prevalence of
positive ESBL producer strain of E. coli in urinary tract infections and this
in turn indicates higher positive ratio of 74.1% in UTIs among patients
admitted in Tehsil Head Quarter (THQ) Hospital, Burewala.
Conclusion: The outcome of our study was that higher prevalence of
ESBL-E. coli (74.1%) was present among parents having UTIs, and
maximum resistance was observed against ampicillin (89%) and
ciprofloxacin (83.7%), however minimum resistance was observed against
ceftriaxone (16%) and nitrofurantoin (14%), means that these drugs are
effective in treatment therapy for UTIs, among patients in THQ, Burewala,
Pakistan.
Beta-Lactamases(ESBL) producing strain of E. coli causing UTIs in
hospitalized patients and to evaluate antimicrobial resistance pattern of
most commonly used antibiotics in treatment therapy, in Tehsil headquarter
hospital in Burewala.
Methods: A total of 150 samples were collected from Patients diagnosed
with urinary tract infections (UTIs), from Tehsil Head Quarter (THQ)
Hospital, Burewala, Pakistan. All samples were streaked on specific media,
MacConkey agar where positive isolates of E. coli showed pink colored
colonies, further confirmation was done by performing some biochemical
tests. ESBL detection of E. coli isolates was confirmed by performing
Double Disc Synergy Test (DDST). In the end antimicrobial resistance
pattern was checked by using some commonly used antibiotics such as
ampicillin, amoxi-clavulanic acid combination, ciprofloxacin, norfloxacin,
TMP-SMX, ceftriaxone and nitrofurantoin and results were observed.
Results: A total of positive 58 samples for E. coli were detected and out of
58 samples, 43 samples were positive for ESBL-E. coli strain and rests
were non-ESBL producers. The results showed higher prevalence of
positive ESBL producer strain of E. coli in urinary tract infections and this
in turn indicates higher positive ratio of 74.1% in UTIs among patients
admitted in Tehsil Head Quarter (THQ) Hospital, Burewala.
Conclusion: The outcome of our study was that higher prevalence of
ESBL-E. coli (74.1%) was present among parents having UTIs, and
maximum resistance was observed against ampicillin (89%) and
ciprofloxacin (83.7%), however minimum resistance was observed against
ceftriaxone (16%) and nitrofurantoin (14%), means that these drugs are
effective in treatment therapy for UTIs, among patients in THQ, Burewala,
Pakistan.
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How to Cite
Pervaz, S., Haneef, K., & Parvez, Z. (2018). Etiology and antimicrobial resistance patterns of Extended
Spectrum Beta Lactamases (ESBL) producing E. coli from urinary
tract infections in Tehsil Head Quarter (THQ) Hospital, Burewala,
Pakistan. Scientific Journal of Medical Research, 2(08), 182–186. https://doi.org/.
Section
Review Article