Effect of Sodium Benzoate on Corticosterone Hormone Level, Oxidative Stress Indicators and Electrolytes in Immature Male Rats

Abstract

Objectives: This study was aimed to determining the effects of sodium benzoate in the levels of corticosterone hormone ,oxidative stress indicators and electrolytes in immature albino male rats in different durations. Methods: 60 immature male rats were used in the main experiment that included three secondary experiments each one of these was included four groups of immature albino male rats, 5 male for each group .In the first secondary experiment G1,G2, and G3 groups were orally given sodium benzoate daily in concentrations of 50,100,200 mg /kg of body weight for one week , whereas in the second and the third secondary experiments G1,G2, and G3 groups were orally given sodium benzoate in the same concentrations daily for two and three weeks respectively. The control groups of the three secondary experiments were given distilled water throughout experiments. After the end of treatments, the level of corticosterone hormone and the indices of the oxidation glotathione and malondealdehyde, as well as levels of sodium and potassium were measured. Results: The results showed A significant increase(P less than 0.05) in the level of corticosterone hormone in both G2 and G3 groups during one weak of treatment and in G1,G2, and G3 groups during two and three weeks of each treatment in comparison with control groups. also there was a significant decrease (P less than 0.05) in the glutathione level accompanied by a significant increase in the malondialdehyde level in both G2 and G3 groups during one week of treatment and also in the G1,G2, and G3 groups during two and three weeks of treatment in comparison with control groups. A significant increase (P less than 0.05) in the sodium level in G1,G2, and G3 groups during each duration of experiment in comparison with control groups. A significant increase (P less than 0.05) in the potassium level in both G2 and G3 groups during one week of treatment and in G1,G2,and G3 groups during two and three weeks of treatment in comparison with control groups. Conclusion: Sodium benzoate caused oxidative stress and have negative effect on corticosterone and electrolytes levels.