Preparation of the Hybrid Nano-ceftriaxone and Study of its Inhibitory Activity against Resistant Bacteria Isolated from Children with Nephrotic Syndrome

Abstract

Objective: Our research aims to train and utilize nano method by two ways to improve antibiotic use in treating blood infections in children with nephrotic syndrome.
Method: The research was done in date between October 2021 and May 2022. In the Pediatric teaching hospital Center for Nephrology/Karbala Health Directorate. A fivemilliliter sample of venous blood was collected from 116 Nephrotic syndrome children
patient and 42 healthy children in age (months to 15 years). The blood culturing procedures were followed, and several analyses were performed, including biochemical parameters and Microbial examination (Bacterial Identification and Antibiotic Susceptibility Test).
Preparation of nanohybrid ZnO-ceftriaxone (drug conjugate with ZnO by sol-gel method), nanohybrid LDH-ceftriaxone (drug conjugate with layers of Mg-AL-No3), were tested then by (FT-IR) Fourier-transform infrared spectroscopy, scanning electron microscope
(SEM), X-ray powder diffraction (XRD), Atomic force microscopy (AFM) analysis. The effect of these nano drugs was examined in isolated bacteria.
Results: The nanohybrid ceftriaxone prepared with ZnO was not effective .while nanohybrid ceftriaxone with layers of Mg-Al-No3 was effective and its conjugation was successful.