Diagnosis of the Causes of Vaginal Candidiasis in Pregnant Women

Abstract

Objective: The aim of the study was to diagnose the types that cause vaginal candidiasis among pregnant women in Karbala.
Methods: Vaginal swabs were collected from pregnant women with symptoms of vaginal infections returning to the Obstetrics and Gynecology Hospital in Karbala. The samples were diagnosed by two methods of direct examination, by culture on plates and by supporting the diagnosis using Vitek device. The samples were cultured on a Sabouraud dextrose agar (SDA) medium. They incubated the dishes at a temperature of 37ºC for 48 to 72 hours to allow the development of colonies. After the emergence of growth on the plates, the samples are examined using the Vitek device, which is characterized by the ability to determine the types of yeasts and the identification of the type of organism. After then, the antifungal activity of free kenaconzole and nanohybrid kenacozole was tested
against Candida prapsilosis.
Results: The results showed that there are nine samples that appeared yeast after culture them in SDA. The results of diagnoses by Vitek showed that four species were C. parapsilosis, three were C. albicans and two werer C. famata. The statistical analysis results showed that the diameter of inhibition increases significantly (p ? 0.05) in the nano hybrid ketoconazole and free kenaconazloe if a  Conclusions: According to the current study, there are no common species of Candida sp. in pregnant women. The success of loading kenconazole on zinc oxide as a carrier of the antibiotic. The activity of the kenacozole and zinc oxide nano-hybrid compound gave a higher inhibition activity than free ketoconazole.